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| Martínez-Cárdenas,Leonardo; Frías-Quintana,Carlos A.; Álvarez-González,Carlos A.; Jiménez-Martínez,Luis D.; Martínez-García,Rafael; Hernández-Almeida,Oscar U.; Bello-Pineda,Javier; Arellano-Méndez,Leonardo U.; Ponce-Palafox,Jesús T.. |
| ABSTRACT Short-tailed pipe fish (Microphis brachyurus) is a freshwater organism with high economic potential for the aquarium hobby, so it is necessary to implement methods to promote its culture through studies of digestive physiology. General activities of acid and alkaline proteases were evaluated, as well as the effect of pH, temperature and inhibitors. The optimal pH of stomach proteases was 2, while the optimal pH of intestinal proteases was 10. Optimal temperature for the acidic proteases was 35 ºC, while for alkaline proteases it was 45 ºC. Thermal stability showed high resistance at 35 ºC for both acid and alkaline proteases (above 100% residual activity). Acid proteases are resistant at pH 2 (50% of residual activity), meanwhile alkaline... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzyme activity; Sygnathidae; PH; Protease inhibitors; Temperature. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000200212 |
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| Merino-Contreras,María L.; Sánchez-Morales,Froylán; Jiménez-Badillo,María L.; Peña-Marín,Emyr S.; Álvarez-González,Carlos A.. |
| ABSTRACT Digestive proteases were partially characterized in sheepshead juveniles, using biochemical and electrophoretic techniques. Results showed higher activity level of the stomach proteases (2.39 ± 0.02 U mg protein-1) compared to the intestinal proteases (1.6 ± 0.1 U mg protein-1). The activity of trypsin, chymotrypsin, leucine aminopeptidase and carboxypeptidase A was also recorded. The optimum temperature of the stomach proteases was recorded at 45 °C, while for intestinal proteases was recorded at 55 °C. Stomach proteases showed less stability to temperature changes than intestinal proteases. An optimum pH of 2 was recorded for stomach proteases with high stability under acidic conditions, while an optimum pH of 9 was recorded for intestinal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquaculture; Inhibitors; Pepsin; Proteases; Zymogram. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000400206 |
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